Part:BBa_J100235:Experience

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AraBAD promoter was tested using Golden Gate Assembly to insert the sequence into E. coli plasmids. Three petri dishes of E. coli colonies were prepared under three different conditions. The positive control contained a promoter known to cause production of red fluorescent protein. The negative control retained the original promoter of the plasmid, which produced green fluorescent protein. The experimental conditions tested the relative red fluorescence with respect to cell concentration in the presence and absence of arabinose of concentration 20µg/mL. Arabinose was the proposed inducer for the promoter. Each experimental condition shown in the graph was run in triplicate using a Synergy scanner to measure fluorescence.